The reliability of the interface made about the quality of the seed lot depends primarily on two components: the accuracy with which the sample represents the lot and the accuracy and precision of the laboratory test.It is observed in many cases that the variations in test results are due to the variation in the sampling.Hence seed sampling is one of the basic component  responsible for the accurate Seed Testing results.Therefore utmost care is required for drawing the sample No matter how accurately the laboratory tests are done, the results can only show the quality of the sample submitted for analysis;consequently the sample should accurately represent the composition of the seed lot.




      The object of sampling is to obtain a representative sample of a size suitable for test.When the sample originates from seed lot,it is expected that the results reflect the average quality of the seed lot.




      A sample is obtained from the seed lot by taking small portions at random from different position of the lot

and combining them.


Seed lot


      It is specified quantity of seed,physically identifiable,in respect of which a seed test certifiacte can be issued.


Primary sample


    Small portion taken from one point in the lot.


Composite sample

     Is formed by combining and mixing all primary samples taken from the lot.


Submitted sample


    Sample submitted to seed testing laboratory .The size of the submitted sample is specified

in the Seed Testing Rules.


Working Sample


      Is a sub sample taken from Submitted Sample in the laboratory,on which one of the quality test is made.


General Principles of Sampling


      Under seed law enforcement programme only trained and experienced officials are authorize to undretake sampling and he has to:


Give notice to such intention to the person from whom he intends to take sample.

Take three representative samples in the prescribed manner and mark & seal.


          * One sample to be deliver to the person from whom it has been taken

          * Second to be sent for analysis to the Seed Analyst of the area.

          . Third to be retained for any legal preceedings.

          . At least two persons should be present and obtain the signature of both the witnesses on from VII of the Seed Act:


Following information should be checked on label




                iii.Lot Number

                iv.Date of Test

                v.Seller's name&addre


In case of certified lots sampler should check Information on seed certification tag:


.    Name & Address of certification agency

.    Kind & Variety

.    Lot No.

.    Name & address of certifird seed producer

.    Date of issue of the certificate & its validity

.    Class & Designation of seed

.   Period during which the seed shall be used for sowing


The seed lot should be so arranged that each individual Or part of the lot is conveniently accessible.



         upon the request of the sampler,the owner shall provide full information regarding  bulking and mixing of the lot.When there is definite evidence of heterogeneity sampling shall be refused.

If the nature of the presentation of the seed lot or container makes it impossible to adequately apply these procedures,then the sampling Shall not be undertaken,and alternative presentation of the seed lot should be sought.

The size of the seed lot should not exceed to the maximum size as prescribed in the rules subject to 5% of tolerance.


Minimum Sampling Intensity For ssed lots in containers


Containers                                    No. of Primary Samples

1-4                                              3 samples from each container


5-8                                              2 samples from each container


9-15                                            1 samples from each container


16-30                                          15 samples in total


31-59                                          20 samples in total


60 or more                                  30 samples in total



      For seed lots in containers smaller than 15kg capacity,containers shall be combined to sampling units not exceeding 100kg and sampling units shall be refarding as containers in the sampling scheme above.


Equipments for Sampling


Sleeve or Stick type trier(Bag trier)


       The tube ans sleeve have open slots in their walls So that when the tube is turned until the slots in the tube and sleeve are In line.Seed can flow into the cavity of the tube,and when the tube is given half turn the opening are close.


Bin tier: It is larger than sleeve tier constructed on the same principle. It is used for sampling from heaps and godowns.

Nobe tier: Pointed tube long enough to reach the center of bag with an oval hole near the pointed end.


Methods for obtaining working samples


A.    Sampling in seed Testing Laboratory


Seed samples received in the seed Testing Laboratory in the seed testing laboratory (submitted sample) are required to be reduced to obtain working samples for carrying out various test.



Soil Divider


   It is simpler divider,built on the same principle as the conical divider, is the soil divider. The channels are here arranged in a straight row instead of a circle as in the conical divider. The soil divider consists of a hopper with attached channels or ducts , a frame to hold the hopper, two receiving pans and a pouring pan.